realtime pcr master mix (Toyobo)
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Toyobo
realtime pcr master mix
Realtime Pcr Master Mix, supplied by Toyobo, used in various techniques. Bioz Stars score: 99/100, based on 11172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sybr+green+master+mix/Realtime+PCR+Master+Mix/custom%40qpk-101%4042736676
Average 99 stars, based on 11172 article reviews
Realtime Pcr Master Mix, supplied by Toyobo, used in various techniques. Bioz Stars score: 99/100, based on 11172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sybr+green+master+mix/Realtime+PCR+Master+Mix/custom%40qpk-101%4042736676
Average 99 stars, based on 11172 article reviews
realtime pcr master mix - by Bioz Stars,
2026-09
99/100 stars
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Expressing:Article Title: Cancer-associated adipocyte promote progression and immunosuppression in triple-negative breast cancer Article Snippet: After determining the concentration and purity, reverse transcription was performed to synthesize cDNA (ReverTra Ace ® qPCR RT Master Mix with gDNA Remover (Code No. FSQ-301), TOYOBO, Japan). .. The mRNA expression levels of adiponectin and the internal control GAPDH were detected on a real-time quantitative PCR instrument using Article Title: Whole Blackcurrant ( Ribes nigrum ) Alleviates High‐Fat Diet Induced‐Obesity and Colonic Inflammation by Modulating the Gut Microbiota in Mice Article Snippet: Total RNA (1000 ng) was reverse‐transcribed into cDNA using an RT Master Mix (#RR036A, Takara Bio Inc., Shiga, Japan) according to the manufacturer's instructions. .. Expression of the target gene was confirmed by amplifying with the 7500 Real‐time PCR system (#4345241, Applied Biosystems, Foster City, CA, USA) using the Article Title: Whole Blackcurrant (Ribes nigrum) Alleviates High-Fat Diet Induced-Obesity and Colonic Inflammation by Modulating the Gut Microbiota in Mice. Article Snippet: Total RNA (1000 ng) was reverse-transcribed into cDNA using an RT Master Mix (#RR036A, Takara Bio Inc., Shiga, Japan) according to the manufacturer’s instructions. .. Expression of the target gene was confirmed by amplifying with the 7500 Realtime PCR system (#4345241, Applied Biosystems, Foster City, CA, USA) using the Article Title: 4632427E13Rik Facilitates Jaw Marrow-Derived Mesenchymal Stem Cells Osteogenesis and Angiogenesis Under Hypoxia Through miR-34a-5p/Aldoa/Hif-1α Pathway Article Snippet: The concentration and purity of total RNA were assessed using the NanoDrop 2000 spectrophotometer (Thermo Scientific). .. For miR-34a-5p quantification, cDNA synthesis and qPCR were performed following the manufacturer’s protocols (Sangon Biotech; Cat. No. B532451 for cDNA synthesis, Cat. No. B532461 for PCR). cDNA was used as a template in quantitative real-time PCR (qRT-PCR) with Control:Article Title: Cancer-associated adipocyte promote progression and immunosuppression in triple-negative breast cancer Article Snippet: After determining the concentration and purity, reverse transcription was performed to synthesize cDNA (ReverTra Ace ® qPCR RT Master Mix with gDNA Remover (Code No. FSQ-301), TOYOBO, Japan). .. The mRNA expression levels of adiponectin and the internal control GAPDH were detected on a real-time quantitative PCR instrument using Real-time Polymerase Chain Reaction:Article Title: Cancer-associated adipocyte promote progression and immunosuppression in triple-negative breast cancer Article Snippet: After determining the concentration and purity, reverse transcription was performed to synthesize cDNA (ReverTra Ace ® qPCR RT Master Mix with gDNA Remover (Code No. FSQ-301), TOYOBO, Japan). .. The mRNA expression levels of adiponectin and the internal control GAPDH were detected on a real-time quantitative PCR instrument using Article Title: Localized Rectal Dextran Sulfate Sodium-Induced Colitis Is Associated with Small-Intestinal Shortening and Gut-Liver Axis-Related Alterations. Article Snippet: RNA concentration and purity were measured with a Qubit 3.0 Fluorometer (Invitrogen, Carlsbad, CA, U.S.A.). cDNA was synthesized from 2 μg of total RNA using a High-Capacity RNA-to-cDNA Kit (Applied Biosystems, Waltham, MA, U.S.A.). .. Quantitative real-time PCR was carried out on the StepOnePlus Real-Time PCR System (Applied Biosystems) using Article Title: Veronicastrum sibiricum (L.) Pennell extract alleviates inflammation-induced muscle atrophy through NLRP3 inflammasome regulation and mitochondrial function restoration Article Snippet: .. RT-qPCR was performed using a Cycler iQTM Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.) and Article Title: Sterol Carrier Protein X (SCP-x) Regulates Cholesterol Transport in the Migratory Locust Locusta migratoria . Article Snippet: .. RT-qPCR was performed using a Bio-Rad Real-Time PCR system with Article Title: Whole Blackcurrant ( Ribes nigrum ) Alleviates High‐Fat Diet Induced‐Obesity and Colonic Inflammation by Modulating the Gut Microbiota in Mice Article Snippet: Total RNA (1000 ng) was reverse‐transcribed into cDNA using an RT Master Mix (#RR036A, Takara Bio Inc., Shiga, Japan) according to the manufacturer's instructions. .. Expression of the target gene was confirmed by amplifying with the 7500 Real‐time PCR system (#4345241, Applied Biosystems, Foster City, CA, USA) using the SYBR Green Assay:Article Title: Cancer-associated adipocyte promote progression and immunosuppression in triple-negative breast cancer Article Snippet: After determining the concentration and purity, reverse transcription was performed to synthesize cDNA (ReverTra Ace ® qPCR RT Master Mix with gDNA Remover (Code No. FSQ-301), TOYOBO, Japan). .. The mRNA expression levels of adiponectin and the internal control GAPDH were detected on a real-time quantitative PCR instrument using Article Title: Localized Rectal Dextran Sulfate Sodium-Induced Colitis Is Associated with Small-Intestinal Shortening and Gut-Liver Axis-Related Alterations. Article Snippet: RNA concentration and purity were measured with a Qubit 3.0 Fluorometer (Invitrogen, Carlsbad, CA, U.S.A.). cDNA was synthesized from 2 μg of total RNA using a High-Capacity RNA-to-cDNA Kit (Applied Biosystems, Waltham, MA, U.S.A.). .. Quantitative real-time PCR was carried out on the StepOnePlus Real-Time PCR System (Applied Biosystems) using Article Title: Veronicastrum sibiricum (L.) Pennell extract alleviates inflammation-induced muscle atrophy through NLRP3 inflammasome regulation and mitochondrial function restoration Article Snippet: .. RT-qPCR was performed using a Cycler iQTM Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.) and Article Title: Mechanistic insights into osteotoxicity induced by early-life lead exposure: Evidence from metabolomics and network toxicology. Article Snippet: One microgram of total RNA was reverse-transcribed into complementary DNA (cDNA) using a reverse transcription kit (FSQ-101, TOYOBO, Japan). .. RT-qPCR was performed using Article Title: Sterol Carrier Protein X (SCP-x) Regulates Cholesterol Transport in the Migratory Locust Locusta migratoria . Article Snippet: .. RT-qPCR was performed using a Bio-Rad Real-Time PCR system with Article Title: Whole Blackcurrant ( Ribes nigrum ) Alleviates High‐Fat Diet Induced‐Obesity and Colonic Inflammation by Modulating the Gut Microbiota in Mice Article Snippet: Total RNA (1000 ng) was reverse‐transcribed into cDNA using an RT Master Mix (#RR036A, Takara Bio Inc., Shiga, Japan) according to the manufacturer's instructions. .. Expression of the target gene was confirmed by amplifying with the 7500 Real‐time PCR system (#4345241, Applied Biosystems, Foster City, CA, USA) using the Article Title: Whole Blackcurrant (Ribes nigrum) Alleviates High-Fat Diet Induced-Obesity and Colonic Inflammation by Modulating the Gut Microbiota in Mice. Article Snippet: Total RNA (1000 ng) was reverse-transcribed into cDNA using an RT Master Mix (#RR036A, Takara Bio Inc., Shiga, Japan) according to the manufacturer’s instructions. .. Expression of the target gene was confirmed by amplifying with the 7500 Realtime PCR system (#4345241, Applied Biosystems, Foster City, CA, USA) using the Quantitative RT-PCR:Article Title: Veronicastrum sibiricum (L.) Pennell extract alleviates inflammation-induced muscle atrophy through NLRP3 inflammasome regulation and mitochondrial function restoration Article Snippet: .. RT-qPCR was performed using a Cycler iQTM Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.) and Article Title: Mechanistic insights into osteotoxicity induced by early-life lead exposure: Evidence from metabolomics and network toxicology. Article Snippet: One microgram of total RNA was reverse-transcribed into complementary DNA (cDNA) using a reverse transcription kit (FSQ-101, TOYOBO, Japan). .. RT-qPCR was performed using Article Title: Sterol Carrier Protein X (SCP-x) Regulates Cholesterol Transport in the Migratory Locust Locusta migratoria . Article Snippet: .. RT-qPCR was performed using a Bio-Rad Real-Time PCR system with Polymerase Chain Reaction:Article Title: Mechanistic insights into osteotoxicity induced by early-life lead exposure: Evidence from metabolomics and network toxicology. Article Snippet: One microgram of total RNA was reverse-transcribed into complementary DNA (cDNA) using a reverse transcription kit (FSQ-101, TOYOBO, Japan). .. RT-qPCR was performed using Article Title: Whole Blackcurrant (Ribes nigrum) Alleviates High-Fat Diet Induced-Obesity and Colonic Inflammation by Modulating the Gut Microbiota in Mice. Article Snippet: Total RNA (1000 ng) was reverse-transcribed into cDNA using an RT Master Mix (#RR036A, Takara Bio Inc., Shiga, Japan) according to the manufacturer’s instructions. .. Expression of the target gene was confirmed by amplifying with the 7500 Realtime PCR system (#4345241, Applied Biosystems, Foster City, CA, USA) using the Article Title: 4632427E13Rik Facilitates Jaw Marrow-Derived Mesenchymal Stem Cells Osteogenesis and Angiogenesis Under Hypoxia Through miR-34a-5p/Aldoa/Hif-1α Pathway Article Snippet: The concentration and purity of total RNA were assessed using the NanoDrop 2000 spectrophotometer (Thermo Scientific). .. For miR-34a-5p quantification, cDNA synthesis and qPCR were performed following the manufacturer’s protocols (Sangon Biotech; Cat. No. B532451 for cDNA synthesis, Cat. No. B532461 for PCR). cDNA was used as a template in quantitative real-time PCR (qRT-PCR) with cDNA Synthesis:Article Title: 4632427E13Rik Facilitates Jaw Marrow-Derived Mesenchymal Stem Cells Osteogenesis and Angiogenesis Under Hypoxia Through miR-34a-5p/Aldoa/Hif-1α Pathway Article Snippet: The concentration and purity of total RNA were assessed using the NanoDrop 2000 spectrophotometer (Thermo Scientific). .. For miR-34a-5p quantification, cDNA synthesis and qPCR were performed following the manufacturer’s protocols (Sangon Biotech; Cat. No. B532451 for cDNA synthesis, Cat. No. B532461 for PCR). cDNA was used as a template in quantitative real-time PCR (qRT-PCR) with |